Fig. 10

Colony forming assay on A549, MDA-MB-231 and Caco-2 cell lines after treatment with 75 µg/mL of pyocyanin extract for 9 days. (A) Representative images of control (upper panel) and cells treated with 75ug/mL of pyocyanin extract (lower panel). Quantification of colonies after treatment with pyocyanin in A549 (B), MDA-MB-231 (C) and Caco-2 (D) represented as colony number/well. Data were expressed as mean ± SEM (n = 3) and statistical significance was assessed by t tests. * Denotes a significant difference compared to control (*< 0.05, **< 0.01, ***<0.001 and ****<0.0001)