Fig. 3

Characterization of Δmyo1-CH2 in high-density fermentation. (a) Fed-batch fermentation. The fermentation of the strain Δmyo1-CH2 was conducted in a 5 L bioreactor using BSM medium. The hLZY bioactivity, total protein concentration in the supernatant, and wet cell weight were assessed. (b) SDS-PAGE analysis of the fermentation supernatant. An equal volume of 1.6 µL of the supernatant was sampled, separated using a 15% SDS-PAGE gel, and then stained with Coomassie brilliant blue