Table 4 Primers used in this study
Name | Sequence (5’–3’) |
|---|---|
tkt-ko-UP1 | AGCCAGCGGATATGAAATG |
tkt-ko-UP2 | TGAACTAATGGGTGCTTTAGTTGAAGAGTGCGAATGGTAGCAACTGAT |
tkt-ko-CR2 | CTGAGAAGCCGTATTCGTTAATGTGCGAATGGTAGCAACTGATTTCTTTTCAATTGTATCCATTATTCATTCAGTTTTCGTGCG |
tkt-ko-DN1 | ATTAACGAATACGGCTTCTCAG |
tkt-ko-DN2 | GCGGGTGATGAATGATTGC |
apt-KO-UP1 | GCAGAAATGAACATGCTGTCTCC |
apt-KO-UP2 | TGAACTAATGGGTGCTTTAGTTGAAGACCCTCTTTCGGGTA ATCCGGTAC |
apt-KO-CR2 | TGCCGTCAAGGTAAGAAAGCTCGATTAAGAAAGCGCCCTC TTTCGGGTAATCCGGTACAATTGTTACGTATTGTTTTATTC ATTCAGTTTTCGTGCG |
apt-KO-DN1 | CGCTTTCTTAATCGAGCTTTCTTACCTTG |
apt-KO-DN2 | GCTTATTTTGCAGCACCATTTTTCG |
perR-KO-UP2 | TGAACTAATGGGTGCTTTAGTTGAAGAGGCTTCTTTTAGTT CATGTGCA |
perR-KO-CR2 | ATGATTTTCTTTTTTCGAACACTCTTGGGGCTTCTTTTAGTT CATGTGCAGCCATCCGTCATGCACCTTTATTCATTCAGTTT TCGTGCG |
perR-KO-DN1 | CCAAGAGTGTTCGAAAAAAGAA |
perR-KO-DN2 | TTCGGCAAACGGAAATGTA |
CR1 | TCTTCAACTAAAGCACCCATTAGTTCAACAAACG |
pHP13-2 | AAGCCTGGGGTGCCTAATGAGT |
ph13-pr | CTCACTCATTAGGCACCCCAGGCTTAATTGACGGCTCAGAGTATCATG |
ribA-1 | ATGTTTCATCCGATAGAAGAAGCACTGG |
Pr-(ribA)-2 | TAAAGCGTCCAGTGCTTCTTCTATCGGATGAAACATTTTGAATTCCTCCTTTTGTCCTTATTGGTTA |
ex5-ribA-2 | CCATCATTATGTATGTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
ex5-ndk-1 | CATACATAATGATGGGGTGGAGAATATGATG |
ex5-ndk-2 | GAATATTGACTCTTGACGGTGAACTTGCTTAATAGATCCAGCCTGCCATAAGCT |
ex5-PvegI-1 | GCAAGTTCACCGTCAAGAGTC |
ex5-PvegI-2 | CGTTAAGCCTTCTTTTGAAAATTTACTTTCCCACATTGCATCCACCTCACTACATTTATTGTAC |
ex5-guaB-1 | ATGTGGGAAAGTAAATTTTCAAAAGAAGGC |
ex5-guaB-2 | CCTAATCTCCTCTAAGTTTATGAAATTGTATAGTTAGGTGATTCTTTTGTAATCTG |
ex5-guaA-1 | ACTTAGAGGAGATTAGGTGACAACCAT |
ex5-guaA-2 | CATGCGGCAATTACCCCTGCCCTTCATCTTATTCCCACTCAATCGTCGCAG |
ex5-gmk-1 | GATGAAGGGCAGGGGTAATTGC |
ex5-gmk-2 | CGACGGCCAGTGAATTTATTCAACCTCCAGCATTTTCTTATATCTT |
pHP13-1 | ATTCACTGGCCGTCGTTTTACAAC |
ex4-guaA-3 | CGACGGCCAGTGAATTTATTCCCACTCAATCGTCGCAG |
ex4-guaB-3 | CCCCTGCCCTTCATCTTATGAAATTGTATAGTTAGGTGATTCTTTTGTAATCTG |
ex4-ribA-3 | TGACGGTGAACTTGCTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
ex4-PvegI-3 | TAATCTCCTCTAAGTTGCATCCACCTCACTACATTTATTGTAC |
ex4-pr | CCATCATTATGTATGTTTGAATTCCTCCTTTTGTCCTTATTGGTTATTA |
ex2-ribA-guaA | TAATCTCCTCTAAGTTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
ex2-ribA-gmk | CCCCTGCCCTTCATCTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
ex2-ribA-guaB | TTTACTTTCCCACATTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
ex2-guaB-3 | AACGACGGCCAGTGAATTTATGAAATTGTATAGTTAGGTGATTCTTTTGTAATCTG |
ex5-ribA-2 | CCATCATTATGTATGTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
ex2-ndk-3 | CGACGGCCAGTGAATTTAATAGATCCAGCCTGCCATAAGCT |
Pr-ndk-oe-1 | AAGGAGGAATTCAAACATACATAATGATGGGGTGGAGAATATGATG |
ndk-oe-2 | CGACGGCCAGTGAATTTAATAGATCCAGCCTGCCATAAGCT |
ribA-002 | CGACGGCCAGTGAATTTAGAAATGAAGTAAATGACCTAGCTTGTTCAT |
guaB-1 | AAGGAGGAATTCAAAATGTGGGAAAGTAAATTTTCAAAAGAAGGC |
guaB-2 | CGACGGCCAGTGAATTTATGAAATTGTATAGTTAGGTGATTCTTTTGTAATCTGTACG |
Pr-2 | TTTGAATTCCTCCTTTTGTCCTTATTGGTTA |
guaA-1 | AAGGAGGAATTCAAAATGACAAAGTTAGTGAATGAAATGATTCTTGTC |
guaA-2 | CGACGGCCAGTGAATTTATTCCCACTCAATCGTCGCAG |
gmk-1 | AAGGAGGAATTCAAAATGAAAGAAAGAGGGTTATTAATCGTTCTC |
gmk-2 | CGACGGCCAGTGAATTTATTCAACCTCCAGCATTTTCTTATATCTTGG |
ndk-1 | AAGGAGGAATTCAAAATGATGGAAAAGACTTTTATCATGGTG |
ndk-2 | CGACGGCCAGTGAATTTAATAGATCCAGCCTGCCATAAGCTG |
AmyE-ex5-UP1 | GGCGTGAATGGGAAAAATAAGAGAG |
AmyE-ex5-UP2 | TTTGTTGAACTAATGGGTGCTTTAGTTGAAGACAGTACCTAAGTAACGGTTGCC |
CR1 | TCTTCAACTAAAGCACCCATTAGTTC |
AmyE-ex5-CR2 | TGCTACTCCATGATACTCTGAGCCGTCAATTCAGTACCTAAGTAACGGTTGCCAATTTGATACGATGTCGGTTATTCATTCAGTTTTCGTGCGGAC |
AmyE-ex5-ex5-1 | AATTGACGGCTCAGAGTATCATGG |
AmyE-ex5-ex5-2 | AAAACTGTATTTCTCGGTCCTCGTTACACCTTATTCAACCTCCAGCATTTTCTTATATCTT |
AmyE-ex5-DN1 | GGTGTAACGAGGACCGAGAAATAC |
AmyE-ex5-DN2 | CTCGCAGAATCAAGTGTTGTG |