Table 3 Strains and plasmids used in this study
Strains or plasmids | Description of genotype | Source |
|---|---|---|
Strains | ||
B. subtilis R (BSR) | B.subtilis 168, araR: para-neo, ribO*, rib+(gsiB), purA*, rpe* | [14] |
BEX5 | BSR/pEX5 | This study |
B13 | BSR/pHP13(spe) | This study |
BSRE1 | BSR, â–³amyE::Pr-ribA-ndk-PvegI-guaB-guaA-gmk | This study |
BSRE2 | BSRE1, Δapt | This study |
BSRE3 | BSRE2, perRL71V | This study |
BSRE4 | BSRE3, Δtkt(tkt0) | This study |
BSRE4/pMX45 | BSRE4/pMX45 | This study |
BSRA | BSR/pEXRA | This study |
BSGA | BSR/pEXGA | This study |
BSGB | BSR/pEXGB | This study |
BSGK | BSR/pEXGK | This study |
BSNK | BSR/pEXNK | This study |
BSAA | BSR/pEX2AA | This study |
BSAB | BSR/pEX2AB | This study |
BSAN | BSR/pEX2AN | This study |
BSAG | BSR/pEX2AG | This study |
BSRNBA | BSR/pEX4RNBA | This study |
BSRBAG | BSR/pEX4RBAG | This study |
BSRNBG | BSR/pEX4RNAG | This study |
BSRNAG | BSR/pEX4RNAG | This study |
BSNBAG | BSR/pEX4NBAG | This study |
BSR1 | BSR, Δapt | [20] |
BSR2 | BSR, Δxpt | [20] |
BSR3 | BSR, ΔhprT | [20] |
BSR4 | BSR, ΔadeC | [20] |
BSR5 | BSR, ΔpucABCDE | [20] |
BSR6 | BSR, ΔyfkN | [20] |
BSR7 | BSR, ΔyunD | [20] |
BSR8 | BSR, ΔyktC | [20] |
BSR9 | BSR, ΔycsE | [20] |
BSR10 | BSR, ΔdeoD | [20] |
BSR11 | BSR, ΔpupG | [20] |
BSRPE1 | BSR1/pEX5 | This study |
BSRPE2 | BSR2/pEX5 | This study |
BSRPE3 | BSR3/pEX5 | This study |
BSRPE4 | BSR4/pEX5 | This study |
BSRPE5 | BSR5/pEX5 | This study |
BSRPE6 | BSR6/pEX5 | This study |
BSRPE7 | BSR7/pEX5 | This study |
BSRPE8 | BSR8/pEX5 | This study |
BSRPE9 | BSR9/pEX5 | This study |
BSRPE10 | BSR10/pEX5 | This study |
BSRPE11 | BSR11/pEX5 | This study |
E.coli | Standard cloning strain | This study |
Plasmids | ||
pHP13 (spc) | Sper | [14] |
pEX5 | pHP13(spe)-Pr-ribA-ndk-PvegI-guaB-guaA-gmk, Sper | This study |
pEX4RNBA | pHP13(spe)-Pr-ribA-ndk-PvegI-guaB-guaA, Sper | This study |
pEX4RBAG | pHP13(spe)-Pr-ribA-PvegI-guaB-guaA-gmk, Sper | This study |
pEX4RNBG | pHP13(spe)-Pr-ribA-ndk-PvegI-guaB-gmk, Sper | This study |
pEX4RNAG | pHP13(spe)-Pr-ribA-ndk-PvegI-guaA-gmk, Sper | This study |
pEX4NBAG | pHP13(spe)-Pr-ndk-PvegI-guaB-guaA-gmk, Sper | This study |
pEXRA | pHP13(spe)-Pr-ribA, Sper | This study |
pEXGA | pHP13(spe)-Pr-guaA, Sper | This study |
pEXGB | pHP13(spe)-Pr-guaB, Sper | This study |
pEXGK | pHP13(spe)-Pr-gmk, Sper | This study |
pEXNK | pHP13(spe)-Pr-ndk, Sper | This study |
pEX2AA | pHP13(spe)-Pr-ribA-guaA, Sper | This study |
pEX2AB | pHP13(spe)-Pr-guaA-guaB, Sper | This study |
pEX2AN | pHP13(spe)-Pr-guaA-ndk, Sper | This study |
pEX2AG | pHP13(spe)-Pr-guaA-gmk, Sper | This study |
pMX45 | Eryr, SM19035-derived plasmid, carrying a complete rib operon | [48] |