Fig. 7

Effects of regulating gapA on 2′-FL production. a Schematic diagram of replacing the original promoter and inserting rare codons after initiation codon of gapA. b Production of 2′-FL in different host strains. The recombinant strains were induced and harvested, then suspended in a bioconversion mixture containing 50 mM fructose and 50 mM lactose. The bioconversions were performed at 37 ℃ and 220 rpm for 8 h