Fig. 4

Effects of different marshalling sequences of five genes in integrated plasmids in host strain S03. a Schematic diagram of P09–P012 plasmids. b 2′-FL production using different integrated plasmids. The recombinant strains RFL09–RFL15 were induced and then suspended in a bioconversion mixture containing 50 mM fructose and 50 mM lactose. The bioconversions were performed for 8 h at 37 ℃ and 220 rpm