Fig. 2
From: Efficient production of cembratriene-ol in Escherichia coli via systematic optimization
Construction of recombinant strains producing cembratriene-ol and its efficient production via optimizing key enzyme expression level. a The titer of cembratriene-ol; b Residual glucose concentration and conversion yield of glucose to cembratriene-ol. cbts* lacked a plastid transit peptide (52 AA, 156 bp) compared with cbts; ggpps, geranylgeranyl pyrophosphate synthase gene; idi, isopentenyl-diphosphate Δ-isomerase gene; dxs, 1-deoxy-d-xylulose 5-phosphate synthase gene; ispA, geranyl diphosphate synthase gene. −, no gene overexpression; +, gene overexpression using pACYCDuet-1; 2+, gene overexpression using pCDFDuet-1; 3+, gene overexpression using pETDuet-1; 4+, gene overexpression using pRSFDuet-1.