Fig. 2

Vector diagram of pET-CmEgt1-CmEgt2 (A) and pET-CmE1b2 (D). The SDS-PAGE analysis of time course experiment of BL21-pET-CmEgt1_Egt2 (B; CmEgt1, 99.4 kDa; CmEgt2_His-tag, 53.3 kDa) and BL21-pET-CmE1b2 (E; CmE1b_His tag, 59.5 kDa; CmEgt2_His tag, 53.8 kDa) (Pre, the sample of fermentative cells before induction; 3–12 h, the sample of fermentative cells during the fermentation after induction; triangle and arrow, pointed out the putative CmE1b and CmEgt2 respectively). Predicted protein structure (C) of CmEgt1 by I-TASSER (the cut site of truncated protein CmE1b was pointed out)