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Fig. 9 | Microbial Cell Factories

Fig. 9

From: Non-destructive quantification of anaerobic gut fungi and methanogens in co-culture reveals increased fungal growth rate and changes in metabolic flux relative to mono-culture

Fig. 9

Mono-culture fluxes in MB on glucose suggest that C. churrovis hydrogenosome pathways differ from widely accepted PFL model. At all timepoints, the ratio of formate to acetate plus ethanol concentrations produced during growth differs significantly from unity (A) (p < 0.0015 for all timepoints). The ratio of formate flux to acetate plus ethanol flux differs significantly from unity during mid-exponential phase growth (48 h, p = 0.0070), but not during late-exponential phase growth (76 h, p = 0.7628) (B), suggesting that PFL may dominate hydrogenosome flux only after substrate is depleted and/or inhibitory metabolites have accumulated. Growth curve included (C) for reference of growth phases; 76 h and before was considered exponential phase, and all timepoints after were considered stationary phase

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Microbial Cell Factories

ISSN: 1475-2859

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