Fig. 2
From: Engineering of Saccharomyces cerevisiae for the accumulation of high amounts of triacylglycerol
Metabolic pathway of TAG synthesis from glucose. Reactions that were investigated in this study and had a positive effect on TAG accumulation are colored. Pathway reactions that were upregulated are shown in green. For Ckb1p, which is not encoding a metabolic enzyme, we assume a repressing effect on the activities of Sct1p and possibly Pah1p through phosphorylation, in the case of Pah1p antagonistic to the Nem1p/Spo7p phosphatase complex (see main text). Competing pathways, which were partially blocked, are depicted in red. The mutated variant of Acc1p, together with an overexpression of DGA1 and the deletion of CKB1, GSY2, ARE2 and TGL3 (framed reactions) resulted in a strain with 65% TAG content. Dashed lines several reactions, straight lines single reactions, G3P glucose-3-phosphate, FFA free fatty acids, DAG diacylglycerol