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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved

Fig. 4

Expression of E. adhaerens CGMCC 6315 PnhA and CnhA in Escherichia coli. (A) Comparison of the NHase gene clusters in E. meliloti CGMCC 7333, V. boronicumulans CGMCC 4969, and E. adhaerens CGMCC 6315. B SDS-PAGE analysis of expression of E. adhaerens CGMCC 6315 NHases in Es. coli. Lane M, protein molecular weight markers (116, 66.2, 45, 35, 25 and 18.4 kDa); lanes 1 and 2, the total and soluble protein from Es. coli control cells; lanes 3 and 4, the total and soluble protein from Es. coli cells expressing E. adhaerens CGMCC 6315 CnhA; lane 5, purified CnhA; lanes 6 and 7, the total and soluble protein from Es. coli cells expressing E. adhaerens CGMCC 6315 PnhA; lane 8, purified PnhA

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Microbial Cell Factories

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