Table 3 Primer sequences of RT-qPCR used for the analyses of inflammatory and anti-inflammatory gene expressions of LPS-stimulated THP-1 macrophage cells
| Â | Specific gene | Primer |
|---|---|---|
Cytokines gene | IL-1β | F-GTGGCAATGAGGATGACTTGTTC R-TAGTGGTGGTCGGAGATTCGTA |
IL-6 | F-AGCCACTCACCTCTTCAGAAC R-GCCTCTTTGCTGCTTTCACAC | |
IL-8 | F-CTGATTTCTGCAGCTCTGTG R-GGGTGGAAAGGTTTGGAGTATG | |
IL-10 | F-GTGATGCCCCAAGCTGAGA R-CACGGCCTTGCTCTTGTTTT | |
TNF-α | F-CTGCTGCACT TTGGAGTGAT R-AGATGATCTG ACTGCCTGGG | |
Housekeeping gene | ACTB (β-actin) | F-ATTGCCGACAGGATGCAGAA R-GCTGATCCACATCTGCTGGAA |
GAPDH (Glyceraldehyde-3-phosphatedehydrogenase) | F-CAACAGCGACACCCACTCCT R-CACCCTGTTGCTGTAGCCAAA |