Fig. 2
Cytotoxicity effects of E. coli KUB-36 metabolites and its individual SCFA on various cancer cells. a MCF10-A normal breast cell, b MCF7 breast cancer cell, c HT-29 colon cancer cell and d THP-1 leukaemia cancer cell. The initial concentration of individual SCFA for cytotoxicity analyses was prepared according to the highest concentration of SCFA (Acetic acid = 23.89 mM; Butyric acid = 3.01; Isobutyric acid = 1.69 mM; Propionic acid = 2.15 mM; Valeric acid = 2.83 mM; Isovaleric acid = 2.78 and Caproic acid = 3.63 mM) that present in E. coli KUB-36 metabolites at 8 h of incubation. Data are plotted as average values of IC50 ± standard error. Different lowercase (a–c) letters indicate significant difference (p < 0.05) between mean scores of each incubation in the column