Fig. 5

Schematic of the whole-cell catalysis of cinnamic acid to cinnamyl alcohol by E. coli BLCS in a biphasic system (a). Effects of volumetric ratio of the organic phase to the aqueous phase on the biosynthesis of cinnamyl alcohol (b). Conditions: 3 mL of 100 mM phosphate buffer (pH 7.5) consisting of 17.4 mM cinnamic acid, 102 mM glucose, wet cells (OD_600nm 50) and 0–3 mL of dibutyl phthalate were mixed together at 200 rpm, 30 °C; the reaction lasted 6 h under the above conditions, with the different letters representing significant differences between the treatment means (p < 0.05)