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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications

Fig. 1

The construction of the GIs-deleted mutants of P. putida KT2440. a The physical localization of the deleted regions on the chromosome of P. putida KT2440. b A pipeline for constructing the GIs-deleted mutants of P. putida KT2440. c Confirmation of the deleted genomic regions in the P. putida mutants by PCR. Lanes: M, DNA marker; 1, the use of the genomic DNA of the control strain P. putida KTU as the template; 2, the use of the genomic DNA of the P. putida mutants as the template; 3, the use of ddH2O as the template

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Microbial Cell Factories

ISSN: 1475-2859

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