Fig. 4
From: Engineering probiotics as living diagnostics and therapeutics for improving human health
Engineering probiotic E. coli strain for the detection of gut inflammation via a thiosulfate sensor. The presence of thiosulfate initiates the phosphorylation of thiosulfate response regulator ThsR and sensor ThsS, which activates the transcription of promoter PphsA. Over-expression of a fluorescent reporter gene under control of the promoter PphsA, the engineered probiotic E. coli strain harboring thiosulfate sensor demonstrated specific response and fluorescence in colon inflammation mouse model