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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Establishing an innovative carbohydrate metabolic pathway for efficient production of 2-keto-l-gulonic acid in Ketogulonicigenium robustum initiated by intronic promoters

Fig. 4

Detection of intronic promoter activity. a–d The images of gfp gene expression in recombinant strains initiated by different promoters. Strains harboring plasmids of (a) pBBR1MCS-2, (b) pBBR-PtufB_gfp (c) pBBR-P1_gfp or (d) pBBR-P2_gfp were photographed with a fluorescence microscope. e The whole cell relative fluorescence intensity (RFU/OD) of different strains was measured using a fluorescence microplate reader. Data in e represents the mean ± SD of 3 replicates

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Microbial Cell Factories

ISSN: 1475-2859

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