The effect of hypoxia on the lipidome of recombinant Pichia pastoris

Table 1 Summary of the macroscopic culture parameters

Culture conditions	DCW (g L⁻¹)	Y_X/S (g_DCW \({\text{g}}_{\text{glc}}^{ - 1}\))	Fab secreted (mg_Fab L⁻¹)	q_Fab (mg_Fab \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	q_EtOH (mmol_EtOH \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	q_Ara (mmol_Ara \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	q_O2 (mmol_O2 \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	q_CO2 (mmol_CO2 \({\text{g}}_{\text{DCW}}^{ - 1}\) h⁻¹)	RQ	C-balance
Normoxia	28.5 ± 0.0	0.58 ± 0.00	5.0 ± 0.2	0.017 ± 0.008	0.001 ± 0.000	n.d.	2.04 ± 0.01	2.00 ± 0.01	1.0 ± 0.0	1.02
Hypoxia	20.5 ± 0.4	0.42 ± 0.00	11.0 ± 0.1*	0.049 ± 0.012*	0.434 ± 0.058	0.048 ± 0.013	1.89 ± 0.05	2.60 ± 0.05	1.4 ± 0.0	0.97
Fluconazole	25.0 ± 0.0	0.50 ± 0.01	5.6 ± 0.1*	0.021 ± 0.004	n.d.	0.001 ± 0.000	2.25 ± 0.05	2.57 ± 0.05	1.1 ± 0.0	0.98
Fluconazole + hypoxia	8.9 ± 0.0	0.28 ± 0.00	3.0 ± 0.1*^†	0.033 ± 0.014	1.181 ± 0.017	0.456 ± 0.009	3.74 ± 0.07	5.60 ± 0.10	1.5 ± 0.0	1.18

Physiological parameters of the P. pastoris strain producing Fab 2F5 grown in normoxic and hypoxic conditions, in the presence or absence of fluconazole, in glucose-limited chemostat cultures at D = 0.1 h⁻¹. Values represent the mean ± SD
DCW dry cell weight, glc glucose, Y _X/S biomass to substrate yield, q specific consumption/product formation rates, RQ respiratory quotient, C-balance carbon balance, n.d. not detected. Glucose and glycerol where measured by HPLC and values were always under the detection level
* p < 0.05 for the t tests compared to the normoxia culture
^† p < 0.05 for the t tests compared to fluconazole culture

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