Fig. 3
From: Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp.
Colony PCR to confirm second crossover event. Colonies were selected, boiled and PCR-amplified with primers Test_F and Test_R, with homology to chromosomal regions just outside the flanking regions. Lanes 1–5 kanamycin sensitive colonies selected following the second crossover event, showing a band size 1.58 kb confirming pduAB deletion. Lane 6 Biolabs 1 kb marker. Lane 7 a PCR result from an un-modified TM242 colony with a 2.2 kb band