Fig. 2
From: Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp.
Colony PCR to confirm the first crossover event. Following re-streaking, five colonies were selected from agar with kanamycin and X-Glu, boiled in 20 µl milliQ water and PCR amplified with Bgl_F and Bgl_R. Following a double-crossover, the plasmid bgl gene would not be present. Lane 1 Biolabs 1 Kb ladder, lane 2 the 1.1 kb control bgl PCR product from unintegrated TM242 pUCG3.8Bgl-pdu, lanes 3–7 no PCR product from colonies selected on kanamycin X-Glu plates