Fig. 8

Production and secretion of AmyM in B. subtilis 168 carrying the out-of-frame translational amyM-gfpmut3 fusion. B. subtilis strains 168 and 168 carrying pDAamyM-gfp were grown in LB (a) or EnPressoB medium (b). SDS PAGE was performed with culture supernatant samples after cultivation times of 5.5, 8, 24 and 32 h