Fig. 1
From: Metabolic engineering of Escherichia coli for the production of cinnamaldehyde
Biosynthesis of cinnamaldehyde and in vitro assay of synthesis enzymes. a Three enzymatic reactions (PAL, 4CL, and CCL) for the biosynthesis of cinnamaldehyde from l-phenylalanine. b In vitro assay of PAL from A. thaliana (AtPAL1, black) and S. maritimus (SmPAL, white) at 30 and 37 °C. c In vitro assay of 4CL and CCL at 30 and 37 °C. Two combinations including (i) 4CL from A. thaliana (At4CL1) and CCR from A. thaliana (AtCCR), and (ii) CCL from S. coelicolor (ScCCL) and CCR from A. thaliana (AtCCR) were mixed with cinnamic acid and cinnamaldehyde production was analyzed. Error bars represent standard deviation of the mean (n = 2)