Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose

Table 1 ALA accumulation in recombinant C. glutamicum expressing endogenous gltX, hemA and hemL

C. glutamicum strains	Expressed genes	Cell biomass (OD₆₀₀)	Glutamate (g/L)	ALA accumulation (mg/L)	PBG accumulation (mg/L)	Heme accumulation (mg/L)
PECX	–	18.84 ± 0.76	1.9 ± 0.14	25.44 ± 1.56	1.26 ± 0.26	0.12 ± 0.05
PEGX	gltX	18.62 ± 0.98	1.85 ± 0.17	20.88 ± 1.34	1.27 ± 0.25	0.14 ± 0.04
PEHA	hemA	19.11 ± 1.56	1.95 ± 0.15	56.89 ± 9.21	3.16 ± 0.23	0.56 ± 0.12
PEHL	hemL	18.24 ± 0.87	2.05 ± 0.24	24.93 ± 2.53	1.21 ± 0.41	0.13 ± 0.08
CGAL	hemA, hemL	17.63 ± 0.46	3.55 ± 0.49	79.84 ± 6.56	9.46 ± 0.54	0.82 ± 0.28
PALX	hemA, hemL, gltX	17.86 ± 0.64	2.75 ± 0.31	64.55 ± 3.61	11.12 ± 0.32	1.05 ± 0.23

Samples were taken and measured until 65 h and cultivation was performed at 180 rpm
40 g/L glucose was added initially
Results are the average of three independent experiments

ISSN: 1475-2859