Fig. 7

Cobamide production by P. freudenreichii in vivo. The UHPLC chromatograms of cobamides extracted and purified from cultures grown under anaerobic (a) and microaerobic (b) conditions, with and without addition of DMBI (100 µM) and/or adenine (Ad; 100 µM). Identity of pseudocobalamin (peak 1) and cobalamin (peak 2) were confirmed by LC–MS/MS. See also Additional file 1: Figure S3