Fig. 7
Determination of dissociation constants (KD values) with different substrates titrated to CYP21A2. Dissociation constants for a medrane, b progesterone and c 17OH-progesterone were determined by titration of bovine CYP21A2 with increasing concentrations of the particular substrate leading to a typical type I shift. Hyperbolic regression resulted in the respective KD values, indicating the extent of the binding affinity towards the enzyme