Fig. 4

HPLC chromatograms of the in vitro biotransformation of medrane with bovine CYP21A2 and Adx or etp1^fd as final redox partner. Substrate conversions of medrane were performed with purified bovine CYP21A2 and a Adx as final electron donor, here with its natural reductase AdR, and b etp1^fd as final electron donor, here with its natural reductase arh1 for 30 min. Steroids were extracted and analyzed by HPLC to verify a selective conversion to premedrol, respectively