Fig. 2
From: Optimization of yeast-based production of medicinal protoberberine alkaloids
Enzyme variant and DNA copy number optimization of S9OMT and CAS for increased canadine titer. a S9OMT. b CAS. Strains listed in Table 1. LC–MS/MS analysis was performed on media supernatant collected after 96 h of cultivation in YNB-DO (2 % dextrose) with 2 mM rac-norlaudanosoline. Error bars represent mean values ±1 SD of at least three biological replicates