Fig. 4

The threonine production by the FACS-selected and randomly-selected mutants. Strain ThrH(pTZL2) was used as the control. The threonine production of FACS-selected and randomly-selected mutants was firstly detected by the rough but fast ninhydrin spectrophotometer method as shown as A, B, respectively. The threonine concentration of the control culture was set as 1. The Top 44 FACS-selected and randomly-selected mutants were further cultured and measured by HPLC shown in a and b, respectively. Data in a and b are the mean and standard deviation of independent triplicates.