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Figure 4 | Microbial Cell Factories

Figure 4

From: Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae

Figure 4

NADPH availability affects yeast cold growth. a CEN.PK2-1C derivatives of the gre3 mutant strain carrying plasmids YEplac195 (URA3; Control, empty plasmid), YEpGDH1 (GDH1) and YEpGDH2 (GDH2) were examined for growth at 30 and 15°C on SCD-Ura agar medium. b CEN.PK2-1C wild-type cells carrying plasmids YEplac181 (LEU2; empty plasmid) or YEpGRE3, which contains the S. cerevisiae GRE3 gene, and YEplac195 (URA3; Control, empty plasmid), YEpGDH1 (GDH1) or YEpGDH2 (GDH2) were pre-grown, diluted and spotted for growth assay at 30 and 15°C as described in the Figure 2. In all cases, a representative experiment is shown.

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Microbial Cell Factories

ISSN: 1475-2859

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