Figure 5

Characterization of rHtrA proteins by circular dichroism. SRCD spectra of rHtrA1 (a) and rHtrA2 (b) are shown at various temperatures: 25°C (solid line), 45°C (large dashed line), 65°C (short dashed line), and 85°C (dotted line). In each case, Δε (molar circular dichroism) at 207 nm was plotted against temperature and this graph is shown as an inset.