Figure 2

Xylose metabolism in S. pastorianus. A. S. pastorianus, carrying gene cassettes (shown on the left; Table 1) coding for T.reesei xylose metabolising enzymes, were grown initially in YEPD. The cells were harvested, washed and resuspened in SC-glucose or SC-xylose media. Various dilutions of the cells were plated onto SC-glucose and SC-xylose agar plates. B. Xylose utilization in S. pastorianus expressing xdh1/xyl1, xi, or the empty vector pRS42H. Xylose concentration (g L⁻¹) in the medium on Day 0, , and Day 12, are shown. C. Alcohol (g L⁻¹) produced by S. pastorianus (CMBS-51) expressing xdh1/xyl1 , xi, , or the empty vector pRS42H, . Fermentations were carried out in YEP supplemented with 50 g L⁻¹ xylose. The values represent the mean of duplicate independent experiments and error bars show the standard deviation from the mean.