Figure 2

SDS-PAGE analysis of purified SUMO-SA2. Lane 1: 3 μl PageRuler™ Prestained Protein Ladder (Fermentas, Vilnius, Lithuania). Lane 2: 25 μl of purified SUMO-SA2 before desalting. Lane 3: same as lane 2, but 10x diluted in sample buffer. Lane 3: 25 μl of purified SUMO-SA2 after desalting. Lane 4: same as lane 3 but 10x diluted in sample buffer.