Figure 4
From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli
Activity of UBPD2QL protease in bacterial cells, SDS electrophoresis in 12% polyacrylamide of bacterial lysates. 1 – molecular mass marker (kDa); 2 and 3 – lysates of E. coli BLD3 (DE3) bacteria transformed with pT7UPDQL2, cultured, induced and not induced with IPTG, respectively. The ubiquitin band is marked with an arrow.